Alg1 Ch1 S1 P47

Alg1 Ch1 S1 P47 Youtube Asparagine linked glycosylation protein 1 homolog (alg1) participates in the initial stage of protein n glycosylation and n glycosylation has been implicated in the process of hepatocellular carcinoma (hcc) progression. however, whether alg1 plays a role in human hcc remains unknown. in this study, the expression profile of alg1 in tumorous and corresponding adjacent non tumor tissues was. To investigate the prognostic value of alg1, immunohistochemistry was performed in another 267 paraffin embedded hcc tissue blocks. the demographics and clinical characteristics of the participants are shown in table s1. correlations between alg1 expression and clinico pathological features in hcc are listed in table s2.

Alg1 Ch1 S1 Pp31 Youtube However, whether alg1 plays a role in human hcc remains unknown. in this study, the expression prole of alg1 in tumorous and corresponding adjacent non tumor tissues was analyzed. the relationship of alg1 expression with clinical features and prognosis of hcc patients was also evaluated using immuno histochemical method. Introduction. in the initial step of n glycosylation, the dolichol linked oligosaccharide (dlo) precursor synthetic pathway occurs on the endoplasmic reticulum (er) membrane, where 14 monosaccharide residues are sequentially transferred onto a dolichyl pyrophosphate (pp dol) carrier molecule to form the core oligosaccharide precursor glc3man9glcnac2 pp dol (burda and aebi 1999; kelleher and. Alg1 encodes a β1,4 mannosyltransferase that catalyzes the addition of the first of nine mannose moieties to form a dolichol lipid linked oligosaccharide intermediate required for proper n linked glycosylation. alg1 mutations cause a rare autosomal recessive disorder termed alg1 cdg. to date 13 mutations in 18 patients from 14 families have. Here we report a new method to purify recombinant alg1 in high yield, and a mass spectral approach for accurately measuring its β 1, 4 mannosyltransferase activity. methods: n terminally truncated yeast his tagged alg1 protein was expressed in escherichia coli and purified by histrap hp affinity chromatography. in combination with lc ms.