Figure 3 From A Novel Microfluidic Platform For High Resolution Imaging

Figure 3 From A Novel Microfluidic Platform For High Resolution Imaging The microfluidic device allowed for imaging cellular migration with high resolution, exhibiting an enhanced migration in hypoxia in comparison to normoxia. this microfluidic device presents itself as a promising platform for the investigation of cellular behaviour in a 3d gel scaffold under varying hypoxic conditions. To still enable high resolution imaging through a liquid interface, we devised a microfluidic system that alternately switches between air–liquid and liquid–liquid interface conditions (figure 3). to demonstrate the high resolution imaging capabilities of the developed system, we show a real time visualization of a p. aeruginosa infection.

Figure 1 From A Novel Microfluidic Platform For High Resolution The actual microfluidic device used in the validation experiment had a 7.2 mm thickness on average, and the pdms thickness below the pc film was ~2.75 mm. figure 3(a) shows microscope images of the microfluidic device. in the bright field image, the central gel region flanked by media channels and pdms pillars to contain the gel are displayed. Results in the numerical simulations of oxygen tension in the microfluidic device with without a film of low diffusion coefficient of oxygen ( d film = 2.0 6 10 2 12 m 2 s 2 1 ), supplying 0%. Figure 2a shows a 3×3 grid of s. & zengerle, r. microfluidic platforms for lab on a chip applications. coster, d. et al. rapid prototyping of high resolution large format microfluidic. Aubry, g., zhan, m. & lu, h. hydrogel droplet microfluidic platform for high resolution imaging and sorting of early larval caenorhabditis elegans. lab chip 15 , 1424–1431 (2015).

Figure 2 From A Novel Microfluidic Platform For High Resolution Figure 2a shows a 3×3 grid of s. & zengerle, r. microfluidic platforms for lab on a chip applications. coster, d. et al. rapid prototyping of high resolution large format microfluidic. Aubry, g., zhan, m. & lu, h. hydrogel droplet microfluidic platform for high resolution imaging and sorting of early larval caenorhabditis elegans. lab chip 15 , 1424–1431 (2015). This novel platform enables high resolution high throughput imaging of small cellular and subcellular features as a population ages in a drug free environment. the high throughput aspect of this platform enabled sampling a large set of animals from a large population, thus acquiring information from a subset with a phenotypic distribution. Recent microfluidic studies coupled with automation provided high resolution imaging of a pair of neurons in a small fov at speeds of 150–900 animals per hour 35 and the whole body of c. elegans.