Ipair Single Cell Immune Sequencing Irepertoire

Ipair Single Cell Immune Sequencing Irepertoire Ipair: our core single cell immune sequencing service. when rna is extracted for bulk repertoire sequencing, information related to the cognate pairing of bcr and tcr chains is lost. our single cell service, ipair, is the first consumer based service specifically designed for capturing physically paired variable regions in t cells and b cells. Ipair relies on our arm pcr technology. with ipair, we use a facs based approach to plate single cells into a 96 well icapture plate. our multiplex primer mixes were developed to balance the amplification of both loci simultaneously in the same pcr well in a low reaction volume. we then sequence the results using the illumina miseq platform.

Ipair Irepertoire S Single Cell Immune Sequencing Service Single cell immune sequencing tell us about your research goals when rna is extracted for bulk repertoire sequencing, information related to the cognate pairing of bcr and tcr chains is lost. ipair is the first consumer based single cell immune sequencing service specifically designed for capturing physically paired variable regions in t cells. 5 easy steps to get started: schedule a meeting with one of our experts to discuss our custom assay, nucleic acid extraction, sequencing, and analysis services. choose your sample input: tissue, cells, ffpe, dna or rna, from human or mouse. pick your sequencing read depth. pool your samples (bulk analysis) or sort your cells (single cell analysis). Irepertoire (256) 327 0948 info@irepertoire schedule a consultation with irepertoire application scientists to see how ipair ipair can advance your research. 2023 irepertoire, inc. irepertoire single cell immune profiling with ipair and ipair • cognate receptor pairings • available for human and mouse tcr alpha, beta. 2 ways to use ipair . coamplify using gene specific primers in the same well as the vdj receptor (human only) amplify with oligo dt primers followed by phenotypic sizing primers in a separate plate. compare: gene specific panel vs. oligo dt panel. gene specific panel.

Single Cell Immune Sequencing Irepertoire Inc Irepertoire (256) 327 0948 info@irepertoire schedule a consultation with irepertoire application scientists to see how ipair ipair can advance your research. 2023 irepertoire, inc. irepertoire single cell immune profiling with ipair and ipair • cognate receptor pairings • available for human and mouse tcr alpha, beta. 2 ways to use ipair . coamplify using gene specific primers in the same well as the vdj receptor (human only) amplify with oligo dt primers followed by phenotypic sizing primers in a separate plate. compare: gene specific panel vs. oligo dt panel. gene specific panel. The ipair single cells sequencing has the ability to describe seven chains from humans or mice at the single cell level. this technique has a higher throughput, lower cost and faster output. in addition, ipair analyzer is developed by irepertoire. Here, we describe a very sensitive method that allows for the amplification of both human tcr alpha and beta chains including additional phenotypic markers from single cells in the same reaction well. we have reduced the rt pcr chemistry as low as 1.5 μl, thus providing a cost effective method to analyze single cells from standard facs.

Ipair Single Cell Immune Sequencing Irepertoire The ipair single cells sequencing has the ability to describe seven chains from humans or mice at the single cell level. this technique has a higher throughput, lower cost and faster output. in addition, ipair analyzer is developed by irepertoire. Here, we describe a very sensitive method that allows for the amplification of both human tcr alpha and beta chains including additional phenotypic markers from single cells in the same reaction well. we have reduced the rt pcr chemistry as low as 1.5 μl, thus providing a cost effective method to analyze single cells from standard facs.