Labeling Of Sa360c315 And Sa360 Cells Expressing Fusion Proteins

Labeling Of Sa360c315 And Sa360 Cells Expressing Fusion Proteins Cells expressing fusion proteins sa360c315 and sa360 were treated according to protocols 2 and 3 (see fig. 3a), as indicated. the resulting membranes were processed as described in the legends to. Figure 6 labeling of sa360c315 and sa360. cells expressing fusion proteins sa360c315 and sa360 were treated according to protocols 2 and 3 (see fig.3a), as indicated. the resulting membranes were processed as described in the legends to figs. 3 and 4. top, topology model of sa360. native cysteine residues are indicated with ac.

Y Fast Enables Specific Labeling Of Fusion Proteins In Living Cells Cells expressing fusion proteins sa360c315 and sa360 were treated according to protocols 2 and 3 (see fig. 3a), as indicated. the resulting membranes were processed as described in the legends to. The cdna for the protein of interest can sometimes be directly cloned into the fp vector. however, in many cases, construction of a fluorescent fusion protein (ffp) will necessitate modification of the protein of interest and or the fp. as described below, placement of an fp can affect the localization and functionality of the protein of interest. Watching biological molecules provides clues to their function and regulation. some of the most powerful methods of labeling proteins for imaging use genetically encoded fluorescent fusion tags. there are four standard genetic methods of covalently tagging a protein with a fluorescent probe for cellular imaging. these use (i) autofluorescent proteins, (ii) self labeling enzymes, (iii) enzymes. The hela cells stably expressing snap egfr were also starved in fetal bovine serum free medium and left for another night to reduce the activity and internalization of the egfr fusion protein. on the day of the experiment, cells were washed with starvation medium containing 0.5% bovine serum albumin.